-the aim of this study is to carried out the Antimicrobial and phytochemical effect on five medicinal plants used as chewing sticks in Owo local government of Ondo State, Nigeria. Methods- The methods used for this study are antimicrobial assay, and phytochemical screening. The aqueous, ethanol and ethyl acetate extracts of the leaves, bark and roots of Distemonantus bentaminaitus , Anogeissus leiocarpus, Garcina kola, Piper guineense and Zanthoxylun Lessmamul for the possible source of antimicrobial and phytochemical constituent. The preliminary evaluation of ethanol and aqueous extracts of the plant exhibits appreciable inhibitory activities against the test organisms, while ethyl acetate extract exhibits low inhibitory activity. the test organisms are Staphylococcus mutan, Neisseria sieca, Lactobacillus salivarius, Actinomycetes viscosus and Streptococcus aureus. Garcina kola, Zanthoxylun Lessmamul, Distemonantus bentaminaitus exhibits the high antimicrobial activity and phytochemical constituent and while Piper guineense and Anogeissus leiocarpus shows the low activity against the test organism. Phytochemical constituent of the plant were also investigated, it was observed that, the plant understudy contain some vital phytochemical, namely, alkaloids, tannins, saponin, steroids, plobatanin, terpenoids, flavonoids and cardiac glycosides.
