ABSTRACT
In order to investigate the effects of Yoyo and Swedish bitters on arylesterase activity in very low density lipoprotein (VLDL), 40 male albino rats were divided into 6 groups; i, 3-week Yoyo bitters. ii, 3-week Swedish bitters. iii, 3-week control. iv, 6-week Yoyo bitters. v, 6-week Swedish bitters, vi, 6-week control. Daily weight of animals were determined and dosage of bitters corresponding to weight of each animal was administered. VLDL was isolated by a simple precipitation procedure and Arylesterase activity was determined. There was no significant difference in the effect of both Yoyo and Swedish bitters on arylesterase activity (P>0.05) as compared with the control suggesting that neither bitters affect the VLDL arylesterase activity,. However, there was an increase in the activity of arylesterase (P<0.05) in the entire 6-week groups compared to the 3-week groups suggesting that increase in age might increase VLDL arylesterase activity which is an antioxidant.
TABLE OF CONTENTS
Page
Title page
Certification
Dedication
Acknowledgement
Table of contents
Abstract
CHAPTER ONE
1.0 Introduction
1.1 Bitter foods
1.1.1 Yoyo bitters
1.1.2 Swedish bitters
1.2 Arylesterase
1.3 Lipoproteins
1.4 Justification
CHAPTER TWO
2.0 Literature review
2.1 Yoyo and Swedish bitters
2.2 Arylesterase
2.3 Lipoproteins
2.3.1 Classification of lipoproteins
CHAPTER THREE
3.0 Materials and method
3.1 Materials
3.1.1 Chemicals and reagents
3.1.2 Apparatus
3.1.3 Biochemical instruments
3.2 Methods
3.2.1 Animals and treatment
3.2.2 Dosage
3.2.3 Sacrifice and blood collection
3.2.4 Isolation of VLDL
3.2.5 Determination of Arylesterase
CHAPTER FOUR
4.0 Results
4.1 Characteristics of experimental groups
4.2 Effects of Yoyo and Swedish bitters on VLDLARE
CHAPTER FIVE
5.0 Discussion and conclusion
5.1 Recommendation
References
Appendix
